Tet-On advanced inducible gene expression system analyzes the biological functions of Trop-2 in vitro

The Tet-On advanced inducible gene expression system in vitro is known for genarating robust expression of the desired gene in target cells. The system offers many advantages over other inducible mammalian gene expression systems, such as high specificity, high inducibility, and high absolute expression levels. In this study, the Tet-On advanced inducible gene expression system was applied to induce the expression of the trophoblast cell-surface antigen 2 (Trop-2) gene in vitro and explore the biological functions of Trop-2. 293/pTet-On-Advanced cell lines were generated, and a recombinant vector containing the Trop-2 gene was constructed and transfected into stable cell lines to improve Trop-2 protein expression. In the presence of doxycycline (DOX), the proliferation assay, transwell assay, and wound healing assay were performed to analyze the efficacy of Trop-2. The results showed that the Tet-On advanced inducible gene expression system was established successfully in the cell line 293, Trop-2 protein level in cells was significantly increased, and Trop-2 could enhance growth, migration, and aggression in the cell line 293. This study suggests that the Tet-On advanced inducible gene expression system can induce the expression of interest genes specifically and artificially in vitro and provides a viable and convenient platform for the study of gene function.