Volume 4 Issue 2
Dec.  2020
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Xueying Wang, Yang Zhou, Linlin Liu, Jinmin Ma, Honglong Wu, Lijian Zhao, Weijun Chen, Shengwen Calvin Li, Qian Ran, Zhongjun Li. Coronavirus disease 2019 (COVID-19): diagnosis and prognosis[J]. Blood&Genomics, 2020, 4(2): 96-107. doi: 10.46701/BG.2020022020120
Citation: Xueying Wang, Yang Zhou, Linlin Liu, Jinmin Ma, Honglong Wu, Lijian Zhao, Weijun Chen, Shengwen Calvin Li, Qian Ran, Zhongjun Li. Coronavirus disease 2019 (COVID-19): diagnosis and prognosis[J]. Blood&Genomics, 2020, 4(2): 96-107. doi: 10.46701/BG.2020022020120

Coronavirus disease 2019 (COVID-19): diagnosis and prognosis

doi: 10.46701/BG.2020022020120
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  • Corresponding author: Zhongjun Li and Qian Ran, Lab Medicine Center, Lab of Radiation Biology, State Key Laboratory of Trauma, Burn and Combined Injury, the Second Affiliated Hospital, Army Medical University, Chongqing 400037, China. E-mails: johnneyusc@gmail.com and louise-rq@163.com
  • Received Date: 2020-08-21
  • Accepted Date: 2020-11-03
  • Rev Recd Date: 2020-09-17
  • Available Online: 2021-07-01
  • Publish Date: 2020-12-30
  • Coronavirus disease 2019 (COVID-19) has become a global pandemic with a high rate of transmission. Currently, there is a lack of vaccines and specific drugs for this newly-emerged virus. Timely diagnosis and treatment, as well as isolation of patients and virus carriers, contribute to the effective prevention and control of this epidemic. This review focuses on early stage COVID-19 diagnosis methods and strategies, highlighting the guiding role of laboratory indicators on treatment strategy formulation, and prognosis assessments.


  • △These authors contributed equally to this work.
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  • [1]
    World Health Organization. Coronavirus disease 2019 (COVID-19)-Situation Report-84[R/OL]. (2020-04-13)[2020-04-13]. https://www.who.int/docs/default-source/coronaviruse/situation-reports/20200413-sitrep-84-cov-id-19.pdf?sfvrsn=44f511ab_2.
    National Health Commission of the People's Republic of China. National-wide Updates of COVID-19 Outbreaks[EB/OL]. (2020-04-13) [2020-04-13]. http://www.nhc.gov.cn/2020.
    Han R, Huang L, Jiang H, et al. Early clinical and CT manifestations of coronavirus disease 2019(COVID-19)pneumonia[J]. AJR Am J Roentgenol, 2020, 215(2): 338-343. doi: 10.2214/AJR.20.22961
    Chen L, Liu HG, Liu W, et al. Analysis of clinical features of 29 patients with 2019 novel coronavirus pneumonia[J/OL]. Chinese Journal of Tuberculosis and Respiratory Diseases (in Chinese), 2020, 43: E005(2020-02-07) [2020-04-13]. DOI: 10.3760/cma.j.issn.1001-0939.2020.0005.
    Zhang MQ, Wang XH, Chen YL, et al. Clinical features of 2019 novel coronavirus pneumonia in the early stage from a fever clinic in Beijing[J]. Chinese Journal of Tuberculosis and Respiratory Diseases (in Chinese), 2020, 43(3): 215-218.
    Song F, Shi N, Shan F, et al. Emerging 2019 novel coronavirus (2019-nCoV) pneumonia[J]. Radiology, 2020, 295(1): 210-217. doi: 10.1148/radiol.2020200274
    Zhang JJ, Dong X, Cao YY, et al. Clinical characteristics of 140 patients infected with SARS-CoV-2 in Wuhan, China[J]. Allergy, 2020, 75(7): 1730-1741. doi: 10.1111/all.14238
    Qin C, Zhou L, Hu Z, et al. Dysregulation of immune response in patients with coronavirus 2019 (COVID-19) in wuhan, China[J]. Clin Infect Dis, 2020, 71(15): 762-768. doi: 10.1093/cid/ciaa248
    Li YX, Wu W, Yang T, et al. [Characteristics of peripheral blood leukocyte differential counts in patients with COVID-19][J]. Chinese Journal of Internal Medicine (in Chinese), 2020, 59(5): 372-374.
    Qu R, Ling Y, Zhang YH, et al. Platelet-to-lymphocyte ratio is associated with prognosis in patients with coronavirus disease-19[J/OL]. J Med Virol, 2020 (2020-03-18) [2020-04-13]. DOI: 10.1002/jmv.25767.
    Liu Y, Gayle AA, Wilder-Smith A, et al. The reproductive number of COVID-19 is higher compared to SARS coronavirus[J/OL]. J Travel Med, 2020, 27(2): taaa021 (2020-02-14) [2020-04-13]. DOI: 10.1093/jtm/taaa021.
    Dhama K, Khan S, Tiwari R, et al. Coronavirus disease 2019-COVID-19[J]. Clin Microbiol Rev, 2020, 33(4): 00020-00028. http://www.researchgate.net/publication/342419581_Coronavirus_Disease_2019_-_COVID-19
    Chen J, Qi T, Liu L, et al. Clinical progression of patients with COVID-19 in Shanghai, China[J]. J Infect, 2020, 80(5): e1-e6. doi: 10.1016/j.jinf.2020.03.004
    Chen N, Zhou M, Dong X, et al. Epidemiological and clinical characteristics of 99 cases of 2019 novel coronavirus pneumonia in Wuhan, China: a descriptive study[J]. Lancet, 2020, 395(20): 30211-30217.
    Yao XH, Li TY, He ZC, et al. [A pathological report of three COVID-19 cases by minimal invasive autopsies][J]. Chinese Journal of Pathology (in Chinese), 2020, 49(5): 411-417.
    Xu Z, Shi L, Wang Y, et al. Pathological findings of COVID-19 associated with acute respiratory distress syndrome[J]. Lancet Respir Med, 2020, 8(4): 420-422. doi: 10.1016/S2213-2600(20)30076-X
    Holshue ML, Debolt C, Lindquist S, et al. First case of 2019 novel coronavirus in the United States[J]. N Engl J Med, 2020, 382(10): 929-936. doi: 10.1056/NEJMoa2001191
    Chan KW, Wong VT, Tang S. COVID-19: an update on the epidemiological, clinical, preventive and therapeutic evidence and guidelines of integrative Chinese-Western medicine for the management of 2019 novel coronavirus disease[J]. Am J Chin Med, 2020, 48(3): 737-762. doi: 10.1142/S0192415X20500378
    Liu C, Jiang ZC, Shao CX, et al. Preliminary study of the relationship between novel coronavirus pneumonia and liver function damage: a multicenter study[J]. Chinese Journal of Hepatology (in Chinese), 2020, 28(2): 107-111.
    Yao N, Wang SN, Lian JQ, et al. Clinical characteristics and influencing factors of patients with novel coronavirus pneumonia combined with liver injury in Shaanxi region[J]. Chinese Journal of Hepatology (in Chinese), 2020, 28(3): 234-239.
    Liu M, He P, Liu HG, et al. Clinical characteristics of 30 medical workers infected with new coronavirus pneumonia[J]. Chinese Journal of Tuberculosis and Respiratory Diseases (in Chinese), 2020, 43(3): 209-214.
    Wu C, Hu X, Song J, et al. Heart injury signs are associated with higher and earlier mortality in coronavirus disease 2019 (COVID-19)[J/OL]. medRxiv, 2020 (2020-2-26) [2020-04-13]. DOI: 10.1101/2020.02.26.20028589.
    Ma LK. What we know so far: COVID-19 current clinical knowledge and research[J]. Clin Med (Lond), 2020, 20(2): 124-127.
    Zhang LP, Wang M, Wang Y, et al. Focus on the 2019 novel coronavirus (SARS-CoV-2)[J]. Future Microbiol, 2020, 15: 905-918. doi: 10.2217/fmb-2020-0063
    Li G, Fan Y, Lai Y, et al. Coronavirus infections and immune responses[J]. J Med Virol, 2020, 92(4): 424-432. doi: 10.1002/jmv.25685
    Lippi G, Plebani M. Procalcitonin in patients with severe coronavirus disease 2019 (COVID-19): A meta-analysis[J]. Clin Chim Acta, 2020, 505: 190-191. doi: 10.1016/j.cca.2020.03.004
    Singhal T. A review of coronavirus disease-2019(COVID-19)[J]. Indian J Pediatr, 2020, 87(4): 281-286. doi: 10.1007/s12098-020-03263-6
    Nishiga M, Wang DW, Han Y, et al. COVID-19 and car-diovascular disease: from basic mechanisms to clinical perspectives[J]. Nat Rev Cardiol, 2020, 17(9): 543-558. doi: 10.1038/s41569-020-0413-9
    Xiong Y, Sun D, Liu Y, et al. Clinical and High-Resolution CT features of the COVID-19 infection: comparison of the initial and follow-up changes[J]. Invest Radiol, 2020, 55(6): 332-339. doi: 10.1097/RLI.0000000000000674
    Tang N, Li D, Wang X, et al. Abnormal coagulation parameters are associated with poor prognosis in patients with novel coronavirus pneumonia[J]. J Thromb Hae-most, 2020, 18(4): 844-847. doi: 10.1111/jth.14768
    Zhou F, Yu T, Du R, et al. Clinical course and risk factors for mortality of adult inpatients with COVID-19 in Wuhan, China: a retrospective cohort study[J]. Lancet, 2020, 395(20): 30563-30566.
    Wu F, Zhao S, Yu B, et al. A new coronavirus associated with human respiratory disease in China[J]. Nature, 2020, 579(7798): 265-269. doi: 10.1038/s41586-020-2008-3
    Lu R, Zhao X, Li J, et al. Genomic characterisation and epidemiology of 2019 novel coronavirus: implications for virus origins and receptor binding[J]. Lancet, 2020, 395(20): 30251-30258.
    Hadfield J, Megill C, Bell SM, et al. Nextstrain: real-time tracking of pathogen evolution[J]. Bioinformatics, 2018, 34(23): 4121-4123. doi: 10.1093/bioinformatics/bty407
    GISAID Initiative. Genomic epidemiology of hCoV-19 2020 [EB/OL]. (2020-03-18)[2020-04-13]. https://www.gisaid.org/.
    Rejmanek D, Hosseini PR, Mazet JA, et al. Evolutionary dynamics and global diversity of influenza a virus[J]. J Virol, 2015, 89(21): 01515-01573.
    Zhao Z, Li H, Wu X, et al. Moderate mutation rate in the SARS coronavirus genome and its implications[J]. BMC Evol Biol, 2004, 4: 21(2020-06-30) [2020-04-13]. DOI: 10.1186/1471-2148-4-21.
    Zhang Z, Shen L, Gu X. Evolutionary dynamics of MERS-CoV: potential recombination, positive selection and transmission[J/OL]. Sci Rep, 2016, 6: 25049(2016-05-05) [2020-04-13]. DOI: 10.1038/srep25049.
    Forster P, Forster L, Renfrew C, et al. Phylogenetic network analysis of SARS-CoV-2 genomes[J]. Proc Natl Acad Sci U S A, 2020, 117(17): 9241-9243. doi: 10.1073/pnas.2004999117
    Kim D, Lee JY, Yang JS, et al. The Architecture of SARS-CoV-2 Transcriptome[J]. Cell, 2020, 181(4): 914-921. doi: 10.1016/j.cell.2020.04.011
    Chen Y, Liu Q, Guo D. Emerging coronaviruses: Genome structure, replication, and pathogenesis[J]. J Med Virol, 2020, 92(4): 418-423. doi: 10.1002/jmv.25681
    Aubriotdelmas B, Laude H. Assembly of coronavirus spike protein into trimers and its role in epitope expression[J]. J Virol, 1990, 64(11): 5367-5375. doi: 10.1128/JVI.64.11.5367-5375.1990
    Beniac DR, Andonov A, Grudeski E, et al. Architecture of the SARS coronavirus prefusion spike[J]. Nat Struct Mol Biol, 2006, 13(8): 751-752. doi: 10.1038/nsmb1123
    Nal B, Chan C, Kien F, et al. Differential maturation and subcellular localization of severe acute respiratory syndrome coronavirus surface proteins S, M and E[J]. J Gen Virol, 2005, 86(5): 80670-80671.
    Neuman BW, Kiss G, Kunding AH, et al. A structural analysis of M protein in coronavirus assembly and morphology[J]. J Struct Biol, 2011, 174(1): 11-22. doi: 10.1016/j.jsb.2010.11.021
    Fehr AR, Perlman S. Coronaviruses: an overview of their replication and pathogenesis[J]. Methods Mol Biol, 2015, 1282: 1-23.
    Chang CK, Sue SC, Yu TH, et al. Modular organization of SARS coronavirus nucleocapsid protein[J]. J Biomed Sci, 2006, 13(1): 59-72. doi: 10.1007/s11373-005-9035-9
    Hurst KR, Koetzner CA, Masters PS. Identification of in vivo-interacting domains of the murine coronavirus nucleocapsid protein[J]. J Virol, 2009, 83(14): 7221-7234. doi: 10.1128/JVI.00440-09
    Cui L, Wang H, Ji Y, et al. The nucleocapsid protein of coronaviruses Acts as a viral suppressor of RNA silencing in mammalian cells[J]. J Virol, 2015, 89(17): 1315-1331.
    World Health Organization. Coronavirus disease (COVID-19) technical guidance: Laboratory testing for 2019-nCoV in humans[EB/OL]. (2020-03-18)[2020-04-13]. https://www.who.int/, 2020.
    Zhu N, Zhang D, Wang W, et al. A novel coronavirus from patients with pneumonia in China, 2019[J]. N Engl J Med, 2020, 382(8): 727-733. doi: 10.1056/NEJMoa2001017
    Tan W, Zhao X, Wang W, et al. hCoV-19/Wuhan/IVDC-HB-01/2019. 01-10-2020 ed[EB/OL]. (2020-03-18)[2020-04-13]. https://platform.gisaid.org/: Global Ini-tiative on Sharing All Influenza Data, 2020.
    Tan W, Ma X, Zhao X, et al. hCoV-19/Wuhan/IVDC-HB-05/2019. 01-10-2020 ed[EB/OL]. (2020-03-18)[2020-04-13]. https://platform.gisaid.org/: Global Ini-tiative on Sharing All Influenza Data, 2020.
    World Health Organization. Pneumonia of unknown cause-China: World Health Organization, 2020[R].
    National Health Commission of the People's Republic of China. Protocol for prevention and control of COVID-19(6th ed)[R]. China CDC Weekly: Chinese Center for Disease Control and Prevention, 2020.
    National Medical Products Administration. Emergency Use Authorizations for COVID-19 Dignosis Kits[EB/OL]. (2020-03-18)[2020-04-13]. http://www.nmpa.gov.cn/WS04/CL2056/376095.htm, 2020.
    US Food & Drug Administration. Coronavirus Disease 2019 (COVID-19) Emergency Use Authorizations for Medical Devices[EB/OL]. (2020-03-18)[2020-04-13]. https://www.fda.gov/medical-devices/emergency-situ-ations-medical-devices/emergency-use-authorizations-covid19ventilators, 2020.
    Authorized Institution for Nucleic Acid Tests in Hubei Province [EB/OL]. (2020-3-10)[2020-04-13]. http://wjw.hubei.gov.cn/bmdt/ztzl/fkxxgzbdgrfyyq/fkdt/202002/t20200210_2022611.shtml.
    COVID-19 Prevention Headquarter of Guangdong Province. Next steps for COVID-19 control: mandatory nucleic acid tests for all patients in the fever clinics[EB/OL]. (2020-03-19)[2020-04-13]. http://wsjkw.gd.gov.cn/zwyw_gzdt/content/post_2887910.html: Health Commission of Guangdong province.
    COVID-19 Prevention Headquarter of Shanghai. COVID-19 prevention at international ports of entry in Shanghai municipal health commission[R]. 2020.
    World Health Organization. Clinical management of severe acute respiratory infection when novel coronavirus (nCoV) infection is suspected: Interim Guidance: Institutional Repository for Information Sharing [R]. 2020.
    Wang W, Xu Y, Gao R, et al. Detection of SARS-CoV-2 in different types of clinical specimens[J/OL]. JAMA, 2020 (2020-03-12) [2020-04-13]. DOI: 10.1001/jama.2020.3786.
    Wu Y, Guo C, Tang L, et al. Prolonged presence of SARS-CoV-2 viral RNA in faecal samples[J]. Lancet Gastroenterol Hepatol, 2020, 20: 30082-30083.
    Handbook of COVID-19 Prevention and Treatment[R]. Hangzhou, China: Jack Ma Foundation, Zhejiang University, The First Affiliated Hospital, Zhejiang University School of Medicine (FAHZU), 2020.
    National Health Commission of the People's Republic of China. Technical guide for laboratory testing of SARS-CoV-2(2nd ed)[R]. China CDC Weekly: Chinese Center for Disease Control and Prevention, 2020.
    Liu L, Liu W, Zheng Y, et al. A preliminary study on serological assay for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in 238 admitted hospital patients[J]. Microbes Infect, 2020, 22(4-5): 206-211. doi: 10.1016/j.micinf.2020.05.008
    Cai XF, Chen J, Li Hu J, et al. A peptide-based magnetic chemiluminescence enzyme immunoassay for serological diagnosis of coronavirus disease 2019[J]. J Infect Dis, 2020, 222(2): 189-193. doi: 10.1093/infdis/jiaa243
    Jia X, Zhang P, Tian Y, et al. Clinical significance of IgM and IgG test for diagnosis of highly suspected COVID-19 infection[J/OL]. medRxiv, 2020(2020-03-12) [2020-04-13]. DOI: 10.1101/2020.02.28.20029025.
    Xiang J, Yan M, Li H, et al. Evaluation of Enzyme-Linked Immunoassay and Colloidal Gold-Immunochro-matographic Assay Kit for Detection of Novel Corona-virus (SARS-Cov-2) Causing an Outbreak of Pneumonia (COVID-19)[J/OL]. medRxiv, 2020(2020-2-27) [2020-04-13]. DOI: 10.1101/2020.02.27.20028787.
    Li B, Feng F, Yang G, et al. Immunoglobulin G/M and Cytokines Detections in Continuous Sera from Patients with Novel Coronaviruses (2019-nCoV) Infection[J/OL]. Available at SSRN 3543609, 2020(2020-01-29) [2020-04-13]. DOI: 10.2139/ssrn.3543609.
    Zhang W, Du RH, Li B, et al. Molecular and serological investigation of 2019-nCoV infected patients: implication of multiple shedding routes[J]. Emerg Microbes Infect, 2020, 9(1): 3.
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