Volume 3 Issue 1
Jun.  2019
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Ziyi He, Shaobin Chen, Lin Yu, Qing Wang, Jiaoli Zou. Establishment of laboratory quality control indicators for NAT on blood donors' samples[J]. Blood&Genomics, 2019, 3(1): 27-33. doi: 10.46701/APJBG.2019012018135
Citation: Ziyi He, Shaobin Chen, Lin Yu, Qing Wang, Jiaoli Zou. Establishment of laboratory quality control indicators for NAT on blood donors' samples[J]. Blood&Genomics, 2019, 3(1): 27-33. doi: 10.46701/APJBG.2019012018135

Establishment of laboratory quality control indicators for NAT on blood donors' samples

doi: 10.46701/APJBG.2019012018135
  • Publish Date: 2019-06-30
  • In order to establish and screen nucleic acid test (NAT) quality control indicators for blood donor samples, CT value of each test item were detected by NAT, internal and external quality controls, the number of unsuccessful mixed samples, the split positive rate, the rate of detection efficiency, and the equipment failure rate were collected. The "instant method" is used to establish the Levey-Jennings quality control chart, the mixed test positive rate, the rate of unqualified sample. Possion distribution is used to establish the quality control chart, and it is easily to operate and monitor NAT effectively. The results displayed that quality control charts were established, including unqualified sample Possion distribution quality control chart, the rate of equipment failure quality control chart, the failure rate of reagent batch and inefficient quality control chart, test result positive of Possion distribution quality control chart, quality control charts of NAT positive Possion distribution probability, the rate of split positive quality control charts, quality control charts of unsuccessful mixed samples, quality control chart of reagents effective rate, internal quality control chart, correctness, and NAT qualitative quality control chart. The study established quality control indicators throughout the whole NAT process, which were able to effectively improve NAT quality control and the efficacy of laboratory management.

     

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