Volume 2 Issue 1
Mar.  2018
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Wei Jiang, Lizhou Jia, Gradimir N. Misevic. One simple and efficient technical method of total RNA extraction[J]. Blood&Genomics, 2018, 2(1): 63-66. doi: 10.46701/APJBG.2018012018006
Citation: Wei Jiang, Lizhou Jia, Gradimir N. Misevic. One simple and efficient technical method of total RNA extraction[J]. Blood&Genomics, 2018, 2(1): 63-66. doi: 10.46701/APJBG.2018012018006

One simple and efficient technical method of total RNA extraction

doi: 10.46701/APJBG.2018012018006
  • Publish Date: 2018-03-30
  • In this article, a convenient and efficient approach has been developed to isolate total RNA from cells. Conventional RNA isolation protocol is time-consuming, so to improve this, a modified method was introduced to eliminate ethanol residue by using a tip complex during the process of RNA extraction. It saves time by isolating RNA in this way and achieves high yields. Additionally, the quality of RNA is guaranteed and verified to be applicable for PCR, cDNA synthesis and reverse transcription PCR.

     

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