Volume 2 Issue 3
Sep.  2018
Turn off MathJax
Article Contents
Shaobin Chen, Ziyi He, Qingkai Chen, Lin Yu, Qing Wang. Performance verification of Roche Cobas s201 nucleic acid test system in Dongguan Blood Center[J]. Blood&Genomics, 2018, 2(3): 171-175. doi: 10.46701/APJBG.2018032018115
Citation: Shaobin Chen, Ziyi He, Qingkai Chen, Lin Yu, Qing Wang. Performance verification of Roche Cobas s201 nucleic acid test system in Dongguan Blood Center[J]. Blood&Genomics, 2018, 2(3): 171-175. doi: 10.46701/APJBG.2018032018115

Performance verification of Roche Cobas s201 nucleic acid test system in Dongguan Blood Center

doi: 10.46701/APJBG.2018032018115
  • Available Online: 2021-06-30
  • Publish Date: 2018-09-30
  • In order to verify the performance of the nucleic acid test (NAT) system, the precision and accuracy of the Hamilton STAR pipetting instrument in both single and pool modes were evaluated using pure water simulated for pipetting. The sensitivity, repeatability, anti-interference and anti-contamination ability of the NAT system (Roche Cobas s201) were evaluated using standard serum diluted to different concentrations. The results showed the precisions of the STAR instrument in single and pool modes were 0.07% and 0.09%, respectively, at the accuracies of 3.33% and 5.66%. The sensitivity coincidence rate of Roche Cobas s201 was 100% at the concentration of 3×LoD (Limit of Detection). The amplification was inhibited at the concentration of 2×LoD for the HBV DNA and HCV RNA when the concentration of hemolysis was greater than 7.5%. Lipemia had no effect, and the anticontamination ability was good. The detection rate in the single mode was higher than the pool mode (t=214.3156, P<0.01). This report suggests that the performance of the NAT system is adequate for laboratory purposes.

     

  • loading
  • 加载中

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Article Metrics

    Article views (1160) PDF downloads(1351) Cited by()
    Proportional views
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return